Abstract: Objective To assess the correlation and consistence of low platelet counts detected with automatic hematology analyzer and flow reference method. Methods Platelet counts in 133 anti-coagulated blood samples with a platelet count of ＜100×10⁹/L were detected with automatic hematology analyzers,Sysmex XE-2100,Mindray BC-5800,ABX Pentra 60,and flow reference method,respectively and compared. Results The platelet counts detected with XE-2100 and BC-5800 correlated well with those detected with flow reference method（r=0.971 7 and 0.960 5,P＜0.001）.Bland-Altman analysis showed that the average value of platelet counts detected with 3 hematology analyzers was-2.26-3.32×10⁹/L.The Kappa coefficient for XE-2100（PLT-I） and BC-5800 was similar when 10×10⁹/L or 20×10⁹/L was used as the platelet transfusion threshold（0.88 vs 0.84 and 0.89 vs 0.89）. Conclusion XE-2100 and BC-5800 show a good correlation and consistence with the flow reference method in counting low platelets.However,hematology analyzers are not accurate enough in detecting low platelet counts for the decision of platelet transfusion in severe thrombocytopenic patients,and the number of platelets should be recalculated with flow reference method.