Abstract: Objective To study the underlying plasticity mechanism of human bone marrow mesenchymal stem cells（hMSC） by analyzing the differentiation of hMSC into sweat glands cells（hSGC） and detecting the changes in gene expression. Methods The HMSC were divided into control group（DM group）,hSGC-induced SG group,EGF10 group and EGF20 group after they were isolated,purified and amplified.Changes in morphology of HMSC were observed,and expressions of genes and proteins in fused HMSC were detected by flow cytometry and RT-PCR,respectively. Results The HMSC could survive in different cultured fluids.The number of HMSC was increased 2d after induced culture,and the long spindle-like HMSC changed into flat hSGC,which was especially significant in SG group.The number of HMSC was significantly increased one week after induced culture.EGF and EGFR,closely related with sweat glands,were significantly expressed in hMSC,and CK7 protein was only expressed in hSGC. Conclusion HMSC,cultured with different media,can differentiate into hSGC at morphologic and molecular levels.The plasticity of hMSC may enrich the theory in regulating repair and rebuilding of skin and sweat glands.