Abstract: Objective To observe the effect of amino acids on postprandial blood glucose level in Kunming mice. Methods Kunming mice were divided into control group and experimental group（15 in each group） according to their fasting blood glucose levels.Mice in experimental group were orally given a mixture of amino acid（2g/kg） and glucose（2g/kg） while those in control group received glucose（2g/kg） only.Blood glucose levels in the animals were measured at 0.5,1 and 2h after intake of glucose. Results The blood glucose levels and the area under AUC at 0.5,1,and 2h after intake of D-leucine and L-leucine were significantly lower in experimental group than in control group,but no significant difference was observed at 0.5h after intake of D-leucine and L-leucine.The blood glucose levels and the area under AUC were significantly lower in experimental group than in control group at 0.5h after intake of D-phenylalanine,but no significant difference was observed at 1 and 2h after intake of L-phenylalanie.The blood glucose levels and the area under AUC were significantly lower in experimental group than in control group at 0.5h after intake of phenylalanine（P＜0.01）,but no significant difference was found at 0.5 and 1h after intake of valine,isoleucine,methionine,threonine,and lysine.The blood glucose levels and the area under AUC were significantly lower in experimental group than in control group at 0.5,1 and 2h after intake of leucine and tryptophan（P＜0.01） and at 0.5h after intake of glutamine and serine,at 0.5 and 1h after intake of glycine,arginine,alanine,cysteine,proline（P＜0.01）,but no significant difference was observed at 0.5h after intake of aspartate,tyrosine,glutamate,histidine and asparagine between the two groups. Conclusion Amino acids,especially leucine,can significantly decrease the postprandial blood glucose level in Kunming mice.