Abstract: Objective To observe the effect of brown adipose tissue（BAT） depletion on body fat and blood lipid induced by high fat diet in obesity mice. Methods Twenty-two male C57BL/6J mice at the age of 4-5 weeks were randomly divided into BAT depletion group and control group,11 in each group.Their body fat mass,body fat,Lee’s index,diet consumption,food efficiency ratio and blood lipid were observed after they were fed with high fat diet for 16 weeks. Results At the end of study,the body fat mass,body fat,Lee’s index and triglyceride level were significantly higher while the food efficiency ratio was significantly lower in BAT depletion group than in control group（P＜0.05）,with no significant difference observed in diet consumption and energy intake between the two groups（P＞0.05）. Conclusion Fat is accumulated,obesity becomes severe and triglyceride level is higher in mice fed with high fat diet when their BAT is depleted,suggesting that BAT plays a certain role in preventing obesity.