Abstract: Objective To study the effect of different hepatic vascular occlusion procedures on growth and metastasis of liver cancer. Methods Twenty-four Kunming mice were randomly divided into suspended operation（SO） group,sham operation group,occlusion of portal triad（OPT） group,and occlusion of portal vein（OPV） group（8 in each group）.Three days after a hepatocellular carcinoma（HCC） model was established via portal vein injection of HCC cells,both portal vein and hepatic artery were clamped or portal vein to the median and left liver lobes was selectively clamped for 60 minutes.Five days after reperfusion,expression rates of proliferative cell nuclear antigen（PCNA） in hepatic replacement area（HRA） and in HCC tissues were measured. Results Eight days after injection of HCC cells from mice via the portal vein,the value of HRA with hepatic lobe occlusion was 7.658±2.552,35.612±4.234 and 9.02±3.006 in control group,OPT group and OPV group,respectively（P＜0.01）.The value of HRA without hepatic lobe occlusion was 8.107±2.003,8.698±3.021 and 8.607±2.304 in control group,OPT group and OPV group,respectively（P＜0.01）.The tumor growth ratio between hepatic lobe occlusion and non hepatic lobe occlusion in HRA was 1,2.82 and 1.1 in control group,OPT group and OPV group,respectively.The positive expression rate of PCNA in HCC tissues was 30%,78% and 45% in control group,OPT group and OPV group,respectively. Conclusion Preservation of hepatic artery flow during portal triad blood inflow occlusion can slow down the growth and metastasis of liver cancer in mice.