Abstract: Objective To study the effect of LRP16 expression on sensitivity of Hela cells to ionizing radiation（IR）. Methods Hela cells were transfected with Eukaryon-expressing plasmids（pcDNA-3.1,pcDNA-3.1-LRP16）,and siRNA,control siRNA,siRNA-374 inhibiting expression of LRP16,and underwent IR to induce DNA damage.DNA damage in plasmid-transfected Hela cells was detected by single cell gel electrophoresis（SCGE）.Viability of Hela cells was assayed with a CCK-8 kit.Effect of inhibiting LRP16 expression on apoptosis of Hela cells was examined by flow cytometry. Results The viability of Hela cells was significantly lower after transfection with plasmid than before transfection with plasmid（P＜0.05）.After treated with etoposide,the number of apoptotic cells was significantly greater in experimental group than in control group（P＜0.05）. Conclusion Over-expression of LRP16 can increase the sensitivity of Hela cells to IR and promote their proliferation.