温度对ACD、CPDA全血ATP作用的研究

Effects of temperature on ATP of ACD-blood and CPDA-blood during blood preservation

摘要: 目的：研究非4°条件ACD、CPDA全血保存过程中ATP变化特点。方法：荧光素-荧光素酶法。结果：4℃、22℃、28℃、33℃条件下ACD、CPDA全血在保存过程中ATP变化随温度的升高而加剧，非4℃条件下同一温度组保存1d后CPDA组与ACD组差异有显著性意义。结论：以ATP值大于1.5μmol/gHb为临界，提出了血液保存的有效期限，及ATP在含腺嘌呤的CPDA全血中的保存效果远较ACD全血为好

Abstract: Objective:To study the ATP of ACD and CPDA-blood during the preservation under non-4°C conditions.Methods:Measure the value of ATP by fluorophotometry.Results:The value of ATP changed at 4°C was compared with those at 22°C,28°C and 33°C during blood preservation. CPDA-blood containing adenine maintained higher ATP than ACD-blood at above temperatures.Conclusion:According to the permitted lowest limit of ATP （1.5 umol/gHb）, we found the new RBC survival periods under 22°C,28°C,33°C.And the CPDA-blood may maintain higher ATP than ACD-blood at above temperatures.