艾恒与传统化疗药物在不同分化程度胃癌细胞株中敏感性的研究

魏宇宁; 高丽丽; 王璐

艾恒与传统化疗药物在不同分化程度胃癌细胞株中敏感性的研究

Study of the sensitivity of the gastric cancer cell lines to oxaliplatin or common chemotherapy drugs

摘要

摘要: 目的: 探讨不同分化程度胃癌细胞对艾恒、5-FU、MMC和VP-16的敏感性。方法: 将不同浓度的化疗药物与两株不同分化程度的胃癌细胞株共同温育,采用MTT方法检测化疗各药物对胃癌细胞增殖的影响。结果: 四种化疗药物对两株胃癌细胞的增殖均具有显著的抑制作用,MKN45细胞株对各化疗药物敏感性顺序为:MMC>VP-16>艾恒>5-FU,而SGC7901细胞株对各化疗药物敏感性顺序为:MMC>艾恒>VP-16>5-FU。中分化的SGC7901对艾恒、MMC、VP-16的敏感性均显著高于低分化的MKN45,而MKN45对5-FU的敏感性高于SGC7901。结论: 不同分化程度的胃癌细胞对药物的敏感性不同。

Abstract: Objective: To investigate the sensitivity of Oxaliplatin,5-FU,MMC and VP-16 in gastric cancer cell lines. Methods: Two gastric cancer cell lines with various degrees of differentiation were incubated with chemotherapy drugs of various concentration.The cytotoxicity was examined by MTT test. Results: All of Oxaliplatin,5-FU,MMC and VP-16 had significant killing effect on human gastric cancer cells in vitro.Chemosensitivity sequence for single drug in poorly differentiated gastric cancer cell line MKN45 was MMC＞VP-16＞L-OHP＞5-FU,and that in moderately differentiated gastric cancer cell line SGC7901 was MMC＞L-OHP＞VP-16＞5-FU.SGC7901 is more sensitive to L-OHP,MMC,VP-16 but MKN45 is more sensitive to 5-FU. Conclusion: The effect of chemotherapy drugs on gastric cancer cells was different among 2 celllines.

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