源于健康人与肝癌患者CIK细胞的生物学特性及抗癌活性的研究

刘岩青; 王悦华

源于健康人与肝癌患者CIK细胞的生物学特性及抗癌活性的研究

Comparison research on biological characteristics and anti-cancer activities of CIK cells from healty persons and liver cancer patients

摘要

摘要: 目的:探讨CIK细胞在肝脏肿瘤的生物免疫治疗中的有效性。方法:①分别提取3名健康献血者和3名肝癌患者的外周血单个核细胞(PBMC),常规诱导出CIK细胞后,动态观察CIK细胞增殖活性和表型变化;用四甲基偶氮唑蓝(MTT)法测定其体外细胞毒活性;并对比研究。②50只BALB/c裸鼠分别在前肢腋下接种SMMC-7721肝癌细胞。10 d后选择荷瘤大小相似的裸鼠30只,全部一次性腹腔注射化疗药物后,随机分成3组:单独化疗组;源于健康人CIK治疗组;源于肝癌患者CIK治疗组,每组10只。单独化疗组注射化疗药物后不再进行任何处理,另2组分别于化疗后的第2、第4、第6、第8、第10天腹腔注射源于健康人CIK细胞和源于肝癌患者CIK细胞进行免疫治疗。结果:①两种来源CIK细胞的生物学特性及抗癌活性无明显差异。②两种来源CIK细胞均可提高化疗的效果。肿瘤受抑制的程度无明显差异,但均明显大于单独化疗组(P<0.05)。结论:两种来源同源细胞因子诱导的杀伤(CIK)细胞,在肝癌的治疗中均可提高化疗的效果。

Abstract: Objective:To observe the changes in phenotype,proliferation activity and cytotoxicity of cytokine induced killer（CIK）cells,and then to investigate the auxiliary therapeutical effects of CIK cells after chemotherapy.Methods: CIK cells were generated by culturing prepheral blood mononuclear cells（PBMC） of three healthy blood donors and three liver cancer patients.Then the changes in the proliferation activity and phenotypes of the cells were observed,and MTT assays were used to determine the cytotoxicity in vitro.The anti-tumor activities of CIK cells were evaluated after chemotherapy in BALB/c nude mice with SMMC-7721 liver cancer respectively.Results: CIK cells from both didn't have obvious differences in phenotype,proliferation activity,cytotoxicit,and auxiliary therapeuticai effects.Conclusion: CIK cells may enhance the effects of the chemotherapy in liver cancer treatment.

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