Abstract: Objective：The purpose of this paper is to clone survivin（SVV）gene from human gastric cancer．Methods：The SVV cDNA was obtained by using RT-PCR method with total RNA extracted from the human gastric cancer cell line，SGC7901．After proved to be correct by sequencing ，recombinant expression plasmid p^RSET-SVV was transformed into E．coli BL21（DE3）．The fusion protein was produced by IPTG induction．Results：Obtained two SVV cDNA clones ，SVV-S₄A and SVV-S1B．The sequence of the previous is identical to that of the well-known SVV，but ，the third exon was missed in the latter．A new anticipated M_r 19500-protein band appeared on SDS-PAGE gel induced by IPTG．Conclusion：human SVV cDNA was cloned and a new splicer is found from human gastric cancer．SVV is highly expressedsioned in E．coli．