用多元PCR检测老年患者院内肺部感染的病原

Detectlon of pathogens in elder patients with nosocomial lung infection by multi ─PCR

摘要: 对30例老年院内肺部感染患者的痰标本分别进行多元PCR、一元PCR和常规培养检测绿脓杆菌、流感杆菌和肺炎链球菌。结果表明：（1）多元PCR的检测结果与一元PCR的完全一致，但前者更快速、简便；（2）PCR阳性检出率为66.67%（20/30），细菌培养的为33.33%（10/30），二者差异显著（P＜0.01）。说明多元PCR特异性好，敏感性强，比一元PCR尤其是细菌培养大大节省时间，更适合检测混合感染的病原。提示多元PCR在临床病原微生物检测中具有优越的价值和广阔的前景。

Abstract: Pseudomonas aeruginosa,hemophilus influenza andstreptococci pneumoniae were detected in sputum samples from 30 elder patients with nosocomial lung infection by multi-PCR,mono-PCR and bacteriological culture. Twenty of the 30 sputum samples were positive for the 3 common pathogens by either multi-or mono-PCR(66.67%) while only 10 samples were positive by culture(33.33%,P＜0.01) .Multi-PCR is time-saving in addition to its advantages of high sensitivity and specificity compared with mono-PCR. Thus,multi-PCR technique seems suitable and promising for the detection of pathogens in sputum samples.