Abstract: Objective: To study the aqueous humor flow rate in chronic hypotony by anterior proliferative vitreoretinopathy （aPVR）, and to demonstrate physiologic mechanisms of the hypotony. Methods:Model of hypotony following aPVR was made by cultured homologous rabbit dermal fibroblasts on pigmented rabbits. The intraocular pressure （IOP） was examined preoperatively and on days 7, 14, 28 and 56 postoperatively. The rate of fluorescein diminished in the aqueous humor was tested postoperatively, and the aqueous humor flow rate was calculated. Results:The average IOP of experimental group was lower than that of control group on days 14, 28 and 56 significantly （P＜0. 01）, and the flow rate of aqueous humor in experimental group reduced remarkably. Conclusion: The hypotony in aPVR was associated with decreased aqueous humor flow rate.