飞行人员代谢综合征患者胰岛素受体第8外显子NsiⅠ位点基因多态性研究

Exon 8 Nsi I site polymorphisms of insulin receptor gene in aircrews with metabolic syndrome

摘要: 目的研究胰岛素受体(INSR)基因多态性与飞行人员代谢综合征(MS)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和基因测序技术检测38名飞行人员MS患者和50名正常健康飞行人员INSR第8外显子NsiⅠ位点基因多态性。结果 INSR第8外显子NsiⅠ位点的基因型和等位基因在飞行人员MS组和正常健康对照组存在统计学差异,INSR基因N2等位基因频率在MS组高于对照组(P<0.01)。结论 INSR第8外显子NsiⅠ位点基因多态性与飞行人员MS相关,N2等位基因可能是一个易感基因。

Abstract: Objective To study the correlation between polymorphisms of insulin receptor（INSR）gene and metabolic syndrome（MS）in aircrews. Methods Genotypes of the INSR gene exon 8 Nsi I site in 38 aircrews with MS and 50 health controls were assayed by polymerase chain reaction—restricted fragment length polymorphism and gene sequencing. Results A statistical difference was found in genotypes and allele frequencies of INSR gene exon 8 Nsi I site in aircrews with MS and health controls.The N2 allele frequency of INSR gene was higher in aircrews with MS than in healthy controls（P＜0.01）. Conclusion Polymorphisms of INSR gene exon 8 Nsi I site are associated with MS in aircrews.The N2 allele of INSR gene may be one of the susceptible genes in aircrews with MS.