Abstract: Objective To evaluate the effect of isotonic weaving suture on multiple strand tendon grafts. Methods Tendons were divided into group I as isotonic weaving suture group, non-isotonic weaving groups II and III. The length and diameter of 4-strand tendons were about 10cm and about 6mm, respectively. Mechanical test was performed after weaving suture of the tendons. Results The stiffness and elastic module of tendon grafts were better in isotonic weaving suture group than in non-isotonic weaving suture group. The stiffness and elastic module of tendon grafts were significantly different between pre-and post-inflection points. Conclusion Multiple strand tendon grafts can be used to reconstruct ACL, which is of great significance in maintaining the biomechanical properties of multiple isotonic strand tendons.