不同培养条件下大鼠背根神经节细胞Nav1.8基因的表达
Expression of Nav1.8 in rat dorsal root ganglion neurons under different culture conditions
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摘要: 目的: 观察体外培养大鼠背根神经节在不同培养条件下的生长情况及Nav1.8 mRNA表达水平,为进一步研究Nav1.8基因的功能提供基础。方法: 取新生SD大鼠背根神经节细胞体外培养,观察胎牛血清(FBS)和马血清(HS),以及外源性神经生长因子(NGF)添加于培养基12 h,24 h,48和72 h突触神经元生长情况,并利用RT-PCR方法观察不同培养条件下Nav1.8mRNA表达水平的变化。结果: FBS+NGF组及HS+NGF组Nav1.8的表达水平明显都高于未添加NGF的FBS组和HS组,FBS组和HS组间比较无明显差异。结论: 培养基中血清成分为FBS或FBS+HS不影响细胞生长及Nav1.8的表达,而NGF可显著促进细胞突触的生长,并上调Nav1.8的表达。Abstract: Objective: To investigate the expression of Nav1.8 in rat dorsal root ganglion（DRG） neurons under different（culture） conditions. Methods: By use of RT-PCR we observed the role of different blood serum and exogenous nerve growth factor（NGF） on the expression of Nav1.8 in rat DRG neurons. Results: NGF treatment can significantly upregulate the expression of Nav1.8 in the FBS+NGF group and HS+NGF group compared with the FBS and HS groups,while the Nav1.8 mRNA level showed no significant deference between FBS and HS groups. Conclusion: Exogenous NGF can upregulate the expression of（Nav1.8） in cultured rat DRG neuron,while serum of FBS or HS do not affect the growth of the neuron and the expression of Nav1.8.
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