Abstract: objective: to establish a reference value of the reticulocytes counts and its subpopulation in the healthy child and adolescence.Methods: The reticulocytes were counted by automated hematology analyzer（ADVIA 120） with oxazine 750 as fluorochrome in 628 the healthy child and adolescence.Results: The Student s t-test showed that there were significant differences（（P＜0.05））between the healthy child group and adolescence group for reticulocyte percentage（RET）,reticulocyte Absolute number（RET#）,High fluorescence reticulocytes（HFR）,middle fluorescence reticulocytes（MFR） and low fluorescence reticulocytes（LFR）.But there were no significant differences（P＞0.05）between male and female in the same group for the 5 parameters.They were 0.0153±0.0054,（68.87±24.21）×10⁹/L,0.8424±0.0541,0.1239±0.0392 and 0.0339±0.0238 in the healthy child group.They were 0.0129±0.0040,（59.37±18.23）×10⁹/L,0.8926±0.0434,0.0933±0.0339 and（0.0141±）0.0132 in the healthy adolescence.Conclusions: Reference value of reticulocytes percentage（RET）,reticulocytes Absolute valume（RET#） and reticulocytes subpopulation were correlated with age and not correlated with sex a in the healthy child and adolescence.reticulocytes count;reference values;child;adolescence