SARS病毒抗体效价测定方法的建立及应用

Method for SARS virus antibodies level determination and its application

  • 摘要: 目的: 建立抗SARS IgG和IgM抗体效价定量测定方法。方法:①在SARS冠状病毒抗体IgG诊断试剂盒和IgM诊断试剂盒的基础上,通过选择IgG、IgM标准血清样本,作系列稀释,建立效价和吸光度标准曲线,采用电子表格获得最佳拟合方程式,应用于标本血清效价计算。②应用该方法测定80例康复期SARS患者血清SARS IgG、IgM抗体及其效价。结果:①取得了良好的拟合方程式,R2均可大于0.99。②80名康复期SARS住院患者血清中SARS病毒IgG抗体61名阳性,效价为31.9±40.0(1.1~264.0),偏度系数为3.677;19例阴性。80名康复期SARS住院患者血清SARS病毒IgM抗体19名阳性,效价为2.59±1.35(1.1~5.5),偏度系数为0.927。结论: ①SARS冠状病毒抗体定性诊断试剂盒具有良好的敏感性和特异性,在选择IgG、IgM标准血清和采用电子表格得出相应的最佳拟合方程式后,可计算出患者血清SARS病毒抗体效价。②SARS病毒抗体阳性的康复期SARS患者血清中的SARS IgG抗体呈偏态分散分布,无明显规律可循。

     

    Abstract: Objective:To establish methods for testing the SARS IgG or IgM antibodies level in patients.Methods:① Based on the diagnosis kits for SARS-coronavirus IgG or IgM antibodies qualitative analysis,we obtained the concentration and absorbency curve of SARS-coronavirus IgG or IgM antibodies and the data fitted equations were derived through serial dilution of the selected standardized serums.The fitted equations were applicable for calculating the SARS-coronavirus IgG or IgM antibodies level in serum.② SARS-coronavirus IgG or IgM antibodies level of eighty convalescent in-patients with SARS were analyzed by these methods.Results:① Favorable fitted equations were obtained the R square of which was more than 0.99.② Sixty one of eighty convalescent in-patients with SARS had IgG antibodies positive,and the relative level of IgG antibodies were 31.9± 40.0,ranging from 1.1 to 264.0 and its skewnessis 3.677.nineteen of eighty convalescent in-patients with SARS had IgM antibodies positive,and the relative level of IgM antibodies were 2.59±1.35,ranging from 1.1 to 5.5 and its skewness is 0. 927. Conclusion:① Based on the favorable sensitivity and specific of the kits,Excel can give the fitted equations which characterized the absorbency and the relative level of IgG or IgM antibodies in the serum.②The distribution of IgG antibodies relative level in the convalescences whose SARS virus antibodies were positive was skew and scatter.

     

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