人γ干扰素基因真核表达载体pZip-γ干扰素的构建
The Costruction of pZip IFN-γ——A eukaryotic expression vector for human interfe ron-γ gene
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摘要: 为构建逆转录病毒载体介导的人γ干扰素(INF-γ)基因真核细胞表达载体,将质粒pGEM-1-IFN-γ用BamHI酶切,分离回收人IFN-γcDNA片段,再定向克隆入逆转录病毒载体pZip·Neo·SV(X)1。经酶切鉴定证实该片段已被正确克隆入pZip·Neo·SV(X)1的BamHI酶切位点,构建成IFN-γ基因真核表达载体pZip-IFN-γ。pZip-IFN-γ的构建成功,为开展IFN-γ基因治疗的研究打下基础。Abstract: The aim of this study was designed to construct a retrovirus vector expvessing human Interferon-γ（IFN-γ）gene in eukaryotic cell. The human IFN-γ cDNA was isloated from pGEM 1 IFN-γ,then it was inserted into retrovirus vector pZip· Neo·SV（X）1. The results of enzymic hydrolysis comfirmed that the human IFN-γ cDNA was correctly cloned to pZip·Neo·SV（X）1. The success in construction of human IFN-γ expression vector makes it possible to carry out further research in gene therapy.
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