C8orf4基因在大肠癌中的表达
The expression of C8orf4 in colorectal cancer
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摘要: 目的: 筛查大肠癌分化相关分子标志物。方法: 应用美国A ffym etrix HG-U133寡核苷酸芯片对不同分化程度大肠癌组织和正常大肠黏膜组织的基因表达谱进行检测。采用肿瘤样品分类法(T-c lass)对各样品进行基因分类。并应用实时荧光定量RT-PCR检测候选基因在不同分化程度大肠癌中的差异表达。结果: T-c lass分析获得分类精度100%的大肠癌分化差异表达基因&ESTs 8个。其中C8orf4基因(chromosom e 8 open read ing fram e4)经非多元统计分析,在大肠癌组较正常黏膜表达下调。实时荧光定量RT-PCR检测该基因在不同分化程度大肠癌组间表达,其标准化表达值与基因芯片该基因探针组信号值相关性分析,呈线性相关。两种方法检测C8orf4基因在不同分化程度大肠癌组表达趋势相符。结论: C8orf4基因与大肠癌分化密切相关,可作为大肠癌分化的候选分子标志物。Abstract: Objective: To identify molecular markers correlated with the differentiation of CRC.Methods: Affymetrix oligonucleotide microarrays HG-U133 were used to monitor gene expression on CRC and paired normal mucosa tissue.The tissue samples of CRC were classified by Tclass（Tumor classification system） in molecular level.The expression difference of candidate genes in different tissues was confirmed by real time FQ-RT-PCR（real time fluorescent quantitative reverse transcription polymerase chain reaction）.Results: 8 genes ＆ ESTs with 100% classification accuracy were obtained by Tclass,in which C8orf4 gene has showed lower mRNA expression in the CRC tissue than in paired normal tissue by multifold statistical methods.The normalized expression levels of C8orf4 were significantly different among CRC with different differentiation grades which was confirmed by real time FQ-RT-PCR,and have linear correlation with its expression in GeneChip.Conclusion: C8orf4 is closely correlated with the differentiation of colorectal cancer and can be regarded as candidate molecular marker.Instestinal tumor;PCR
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