Abstract: Objective To observe the effect of RhoB on NF-κB transcriptional activity in human pancreatic cancer SW1990 cells. Methods Transcription activity of genes was detected by double-luciferin and RhoB protein was detected by Western blot 24 h after zero-load plasmid（pcDNA3）,wild-type RhoB over-expression plasmid（RhoB-wt）,RNA interference control plasmid（RNAi-control）,RhoB RNA interference plasmid（RhoB-RNAi）,RhoB-activated plasmid（RhoB-V14） or RhoB-inactivated plasmid（RhoB-N19）,NF-κB reporter gene plasmid（NF-κB-luc）,and intra-reference plasmid（pRL SV40-luc） were transfected into SW1990 cells. Results The over-expression and activation of transfected RhoB（0.8μg） could inhibit the NF-κB reporter gene activity in SW1990 cells by 54% and 21% compared to the control group.The transcriptional activity of NF-κB was 1.5-fold higher in SW1990 cells transfected with RhoB than in the control group. Conclusion RhoB can inhibit the transcriptional activity of NF-κB in pancreatic cancer SW1990 cells.