实时荧光定量PCR检测乙型肝炎病毒DNA的临床应用
The application of real-time FQ-PCR technique in detection of HBV-DNA
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摘要: 目的: 探讨实时荧光定量PCR(FQ-PCR)检测HBV-DNA在乙型肝炎病毒(HBV)感染的诊疗中的应用价值。方法: 用实时荧光定量PCR(FQ-PCR)方法定量检测523例患者的血清HBV-DNA含量(临界值为500cop ies/m l),同时用ELISA法测定HBV血清标记物。结果: 经FQ-PCR检测,116份HbsAg,HbeAg,HbcAb,ProS1-Ag都阳性的标本,HBV-DNA阳性率为98.7%,平均拷贝数为1.87×108copy/m l,显著高于其他组(P<0.05)。HbsAg,HbeAg,ProS1-Ag一项或多项阳性者的HBV-DNA阳性率和平均拷贝数显高于三项抗原全阴者。结论: FQ-PCR可以快速而准确地检测HBV-DNA拷贝数。HBV-DNA可准确、灵敏地反映HBV复制。与HBV血清标记物联合应用可使诊断更准确,治疗更合理。Abstract: Objective:To exqlore the value of HBV-DNA copies detected by real-time fluorescence quantitative PCR（FQ-PCR） in the diagnosis and therapy of HBV infection.Methods:523 patients’ serum HBV-DNA were detected by real-time FQ-PCR.The cutoff is 500 copies/ml.Their serological markers of HBV were detected by ELISA method.Both results of HBV-DNA and serological markers of HBV were compared.Results: In the 116 HbsAg（+）,HbeAg（+）,HbcAb（+） and ProS1-Ag（+） samples,HBV-DNA positive ratio and mean of copies are 98.7% and 1.87×10~8copies/ml.They are much higher than that of other groups（P＜0.05）.The HbsAg（-）,HbeAg（-）,ProS1-Ag（-） group’s positive ratio and copy number of HBV-DNA are much lower than that of HbsAg（+） and/or HbeAg（+）,and/or ProS1-Ag（+） group（P＜0.05）.Conclusion:FQ-PCR is a fast and accurate technique to quantitative the serum HBV-DNA.HBV-DNA can reflect HBV replication accurately.Combination the HBV-DNA and the serological markers of HBV would make the diagnosis and therapy of HBV infection more reasonable and efficient.
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