Abstract: A procedure in preparation of goat antiserum to rabbit IgG is presented. The rabbit IgG was precipitated with saturated amminium sulphate and purified by DEAE cellulse chromatography. During immunization, various immune methods were used, including injections at multiple subcutaneous and these into the lymphnodal, and intravenous or intraperitoneal injections. For the first immunization, a dose of 50mg rabbit IgG. which had been emulsified in complete Freund’s adjuvant, was injected into multiple subcutaneous sites on the neck, of a male goat aged one year. Thereafter, the boost immunizations were performed at one to two months intervals with seven injections. Except for intraperitoneal and intravenous injections, IgG was emulsified in incomplete Freund’s adjuvant prior to the injections. Out of seven boosters, four were injected per multiple subcutaneous routes in a dose of 35—50mg IgG; and the rest, injected per into the lymphnodes together with multiple subcutaneous, intraperitoneal and intravenous injections in doses of 36mg, 50mg and 20mg respectively, seven days after the last boost injection antiserum was harvested With this immunization schodure, antiserum of higher titer has been obtained.