外周血增殖的T细胞CD28分子表达和自身反应性

CD28 molecular expression and autoimmunity in human peripheral blood T cells activation by SEB

摘要: 目的：SEB诱导增殖的外周血T细胞是否具有正常T细胞的功能。方法：用超抗原刺激淋巴细胞生长；用流式细胞测定技术检查细胞表面CD4，CD8，CD28，TCD16的抗原表达量；加入同源，异源抗原检查自身反应性。结果：SEB能诱导外周血CD4⁺T细胞的增殖。随CD4⁺T细胞的增加，CD28分子表达上升。外周增殖的CD4⁺T细胞与胸腺内分化的T细胞相同，97%以上的CD4⁺T细胞都表达CD28分子。但对CD16阳性的抗原提呈细胞的依赖性下降，并显示对自身抗原的识别能力增强。结论：外周活化的CD4⁺T细胞与胸腺内分化的CD4⁺T细胞功能不同。。

Abstract: Objective: We examined the characteristics of human peripheral blood T cells activation by superantigen B（SEB）, and try to know if the proliferous T cells are as same as entrothymic T cells.Methods: We analyzed CD4, CD8, CD16 and CD28 molecular expression and autoimmunity of proliferous T cells by flow cytometry and cell culture. Results: Most of CD4⁺ T cells expressed CD28 molecular The CD4⁺CD8⁺T cells are different from extrothymic T cells. The dependence for T cells to APC exhibited decrease and showed autoimmunity against to isogenous antigen. Conclusion: The function of CD4⁺T cells activation in peripheral blood are different from the function of extrothymic T cells.