灯盏花素对肝缺血再灌注损伤生化指标的影响
Effect of breviscapine on biochemistry in hepatic ischemia-reperfusion injury
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摘要: 目的: 研究灯盏花素对肝缺血再灌注损伤生化指标的影响。方法: 健康家兔32只,复制肝缺血再灌注损伤模型。随机分为假手术组,模型组,灯盏花素(B re)小剂量用药组和灯盏花素大剂量用药组,每组8只。观察缺血前,缺血45m in、再灌注30和60m in时血清超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-PX)、谷丙转氨酶(ALT)活性和丙二醛(MDA)含量及灯盏花素对不同时限上述指标的影响。结果: 灯盏花用药组的SOD、MDA、GSH-PX和ALT在再灌注的各时限与对照组比较均有显著或非常显著差异(P<0.05或P<0.01)。结论: 灯盏花素能通过抑制氧自由基的生成,增强氧自由基的清除,对肝缺血再灌注损伤起着良好的抗脂质过氧化作用。Abstract: Objective:To explor the role of breviscapine （Bre） on dynamic changes of lipid peroxidation in hepatic ischemia-reperfusion injury（HIRI）.Methods:The HIRI model was used.Thirty-two rabbits were randomly divided into control group（（n=8））,model group（n=8）,Bre（5mg） group（n=8） and Bre（7mg） group（n=8）.The superoxide dismutase（SOD） activity,malondialdehyde（MDA） content,glutathione peroxidase（GSHPX） and alanine aminotransferase（ALT） activity in serum were observed before ischemia and at ischemia 45 min,reperfusion 30min,reperfusion 60 min.Results:The SOD activity,GSH-PX activity MDA,content and ALT activity of Bre group,as compared with model group,showed significant differences（（P＜0.05） or P＜0.01） at total time points of reperfusion.Conclusion:Bre has notable anti-lipid peroxidation effect on HIRI,which is due to its inhibiting the generation of oxygen free radicals and its strengthening scavenging of oxygen free radicals.
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