十种蔬菜冷冻干燥制品“赛金”对生长期大鼠的营养作用

Nutritional effect of ten different kinds of freeze-drying vegetables mixture-“Sai Jin” in growing rats

  • 摘要: 目的: 观察10种不同蔬菜经冷冻干燥处理后制备的食品“赛金”对生长期大鼠营养生理功能的影响。方法: 18只断乳大鼠,分为对照组和“赛金”组,单独喂养在代谢笼中,饲喂人工半合成饲料。“赛金”组饲料中由于添加了6%的“赛金”,故将对照组饲料做相应的调整,使两组饲料的能量、蛋白质、粗纤维及其它营养素的含量均保持一致。采用对喂法,使各组大鼠进食量相同。饲养4 0d时分别收集大鼠新鲜粪便,测定其中双歧杆菌(B)、乳酸杆菌、大肠杆菌(E)等菌群水平并计算B/E比值。4 5d后断头取血,测定各项血液学参数和生化指标。结果: 两组大鼠的食物转化率、血浆总蛋白、白蛋白及球蛋白水平均十分接近,血清氨基酸成分与对照组大体相同,说明食用蔬菜组的营养效果与对照组总体差异不大。但“赛金”组大鼠血胆固醇水平显著低于对照组,血清赖氨酸(LYS)、鸟氨酸(ORN)、色氨酸(TRP)显著低于对照组;“赛金”组大鼠粪便中双歧杆菌与大肠杆菌的比值(B/E)明显高于对照组;结论: 饲料中添加6%的蔬菜冷冻干燥制品(赛金)有降低大鼠血胆固醇的作用,并有助于改善肠道菌群。由于蔬菜共同的营养特征是缺乏赖氨酸、色氨酸等必需氨基酸,提示在日常膳食增加蔬菜摄入量的同时,要注意富含优质蛋白质的动物性食物和豆类的摄入,以保证营养均衡。

     

    Abstract: Objective: To observe influence of ten different kinds of freeze-drying vegetables mixture-”Sai Jin” on nutritional status in growing rats. Methods: Eighteen weanling rats were divided into control and ”Sai Jin” groups,fed in metabolic cages individually with semi-synthetic diets. Because of 6% supplement of ”Sai Jin” in diet of experiment group,the constitutes in diet of control group had to be adjusted,so that energy,protein,fiber and other nutrients in two groups may well be matched. Pair fed method was used to keep same intake in two groups. At the 40th day,all the feces were collected to measure flora in feces cultivation such as Lactobacillus,Escherichia coli (E),Bifidobacterium (B) and calculate ratio of B to E. At the 45th day rats were killed by decapitation under ether anaesthesia. Blood were taken for analysis. Results: Food conversion rate, total protein,albumin,and globulin in plasma were nearly similar between two groups. There was no much difference in hematological parameters and some biological indexes between two groups statistically. However,serum cholesterol in rats of ”Sai Jin” group was significantly lower than that of control,meanwhile,the ratio of B to E in feces cultivation was obviously higher compared with control. Composition and contents of serum amino acids in two groups were similar except for lower level of lysine,ornithine and tryptophan in ”Sai Jin” group. Conclusions: Supplement of 6% ”Sai Jin” in diet may decrease blood cholesterol in rats and improve intestinal flora. It was suggested that in order to get a balanced nutrition,intake of sufficient high quality protein from both animal and bean is of importance when increase vegetables intake in diet because lack of some of essential amino acids is a common characteristic of vegetables.

     

/

返回文章
返回