HPCE法测定生黄芩、酒黄芩中黄芩苷含量

Determination of baicalin in Radix Scutellariae and stir-bake with wine Radix Scutellariae by HPCE

摘要: 目的: 建立中药饮片生黄芩、酒黄芩中黄芩苷含量测定的毛细管电泳法。方法: 毛细管电泳法紫外检测生黄芩和酒黄芩中黄芩苷的含量, 以 40mmol/L硼砂缓冲液 (pH 8.5)为电泳介质, 未涂层融硅毛细管 (50 μm× 47cm), 有效分离长度 40cm, 压力进样 17kPa·s, 2 5kV恒压电泳, 检测波长为 2 80nm。结果: 黄芩苷的线性范围在 0.05～0.80mg/ml,回归方程Y =2.1335X-0.0577,r=0.9991,平均回收率 98.96%, n=5, RSD =1.0 2%。结论: 本方法快速、简便、结果准确, 重现性好, 可用于生黄芩、酒黄芩中黄芩苷的含量测定。

Abstract: Objective:To develop a method for the determination of baicalin in Radix Scutellariae and stir-bake with wine Radix Scutellariae by HPCE. Methods: Separation was carried out in an uncoated fused silica capillary 50 μm×47 cm, effective length 40cm. Meanwhile a running voltage 25 kV, 40 mmol/L borax （pH 8.5） buffer and a UV detector at 280 nm adopted. Results: The calibration cruve showed good linearity over the range of 0.05～0.80mg/ml.Regression equation was Y=2.133 5X-0.057 7, r=0.999 1.The average recovery rate was 98.96%,n=5,RSD=1.02%. Conclusions: The method is rapid, simple and accurate with good repeatability and it can be used to determine baicalin.