Abstract: Objective: To develop a rapid and reproducible method in strain identification for Trichophyton rubrum. Methods: Two novel tandem repeat subelements（TRSs）,TRS-1 and TRS-2,located in the Trichophyton rubrum rDNA nontranscribed spacer（NTS） were amplified from 63 strains of Trichophyton rubrum. Results: Specific amplification of TRS-1（6 type） and TRS-2（2 type） produced strain-characteristic bandinding patterns. Conclusions: This new method of typing Trichophyton rubrum will provide a rapid, stable molecular method about pathogenesis and epidemiology of this fungus.