Abstract: Objective To analyze the immune function and myocardium enzyme in officers and soldiers of a division who developed stress disorder when they were helping the people to tide over the disaster in Wenchuan earthquake area,and to evaluate their mental and physical health. Methods The officers and soldiers were divided into positive post-traumatic stress disorder（PTSD） group（A）,negative PTSD group（B）,and normal control group（C）.Their T,B,and NK lymphocyte subpopulations were detected and their myocardium enzyme levels including AST,CK,CKMB and LDH were measured,which were compared with those in the normal control group. Results The immune function was with normal range in 3 groups.The number of CD3⁺ and CD3⁺ CD4⁺ T lymphocytes was greater in group A than in group B,and was smaller in group B than in group C（P＜0.01）.The number of CD4/CD8 T lymphocytes was smaller in groups A and B than in group C（P＜0.01）.The number of CD19⁺ B lymphocytes was significantly smaller in group A than in group B（P＜0.05）.The number of CD19⁺ B lymphocytes in group B and the number of CD3^-CD56⁺ NK lymphocytes in group A were significantly greater than in group C（P＜0.01）,indicating that the immune function of the officers and soldiers was abnormal.The serum AST,CK,CKMB and LDH levels were significantly higher in groups A and B than in group C（P＜0.01）,indicating that the cardiac function of officers and soldiers with positive or negative PTSD in the front disaster relief line was impaired. Conclusion Stress disorder can lead to abnormal immune function and enzyme metabolism in myocardium of those with PTSD.