瘦素对大鼠胰岛细胞胰腺-十二指肠同源盒基因-1表达的调节作用

Effect of leptin on PDX-1 gene expression in isolated rat islets

摘要: 目的:研究不同浓度瘦素对大鼠胰岛细胞PDX-1基因表达的影响,在基因转录调节因子水平探讨其在肥胖2型糖尿病发病中的作用。方法:不同刺激浓度瘦素(0 ng·mL^-1、10 ng·mL^-1、25 ng·mL^-1、50 ng·mL^-1、102ng·mL^-1、103ng·mL^-1)与葡萄糖浓度分别为2.2 mmol·L^-1、5.5 mmol·L^-1、11.1 mmol·L^-1的RPM I1640营养液共培养24 h后,提取细胞总RNA。运用RT-PCR技术检测PDX-1基因表达的变化。结果:葡萄糖浓度为2.2 mmol·L^-1、5.5 mmol·L^-1、11.1 mmol·L^-1时,各种浓度的瘦素对PDX-1基因的表达均无明显影响。结论:瘦素对PDX-1表达无明显调节作用。

Abstract: Objective: To investigate the effect of different concentrations of leptin on PDX-1 gene expression in isolated rat islets.Methods:Leptin was added to isolated islets at final concentrations of 0,10,25,50,102,103 ng·mL^-1 to media containing 2.2 mmol·L^-1（low）,5.5 mmol·L^-1（normal） and 11.1 mmol·L^-1（high） glucose.All islets samples were harvested after incubation for 24 h,and total RNA was extracted for a single series for PDX-1 RT-PCR.Results: There was no change in PDX-1 gene expression with different concentrations of leptin with glucose of 2.2 mmol·L^-1,5.5 mmol·L^-1 and 11.1 mmol·L^-1.Conclusion: Leptin has no significant effect on PDX-1 gene expression.