Abstract: Objective: We use the chitoson polymer microsphere carried with carboplatin treat cultured C₆ glioma cell in vitro. Through the morphological observation, we went to demonstrate the relationship between drug and C₆ glioma cell. Methods: We use reversed microscope and scanning electron microscope to observe chitoson polymer microsphere carried with carboplatin treat cultured C₆ glioma cell in vitro. Results: We find drug microspheres have a good appearance and very stable structure. The ruin rate of drug microspheres is lower than 2% after two weeks. Under the scanning electronic microscope we also observed that the microsphere with carboplatin take off its coat first and then appears many micro holes on their surface. Apoptosis were demonstrated by observation of scanning electronic microscope, Giemsa staining and FCM. Conclusion: Remarkably, the drugs released from microspheres through these holes, but the skeleton of polymer microspheres keeps entirety. Drug microsphere can inhibit cultured C₆ glioma cells in vitro.