FHL2与Id蛋白相互作用的表位分析

Epitope analysis of interaction between FHL2 and Id proteins

摘要: 目的: 鉴定FHL₂与Id家族蛋白成员之间的相互作用及分子表位。方法: GST-pulldown方法检测。结果: FHL₂与Id家族的4个成员蛋白均存在直接的相互作用关系,FHL₂蛋白中的第二个LIM结构域在FHL₂/Id相互作用中是必需的,Id蛋白N端结构区域在介导FHL₂/Id相互作用中是必需的。结论: FHL₂是一个新识别的Id蛋白广谱相互作用因子,FHL₂可能参与Id介导的多种生物学效应以及肿瘤发生与进展。

Abstract: Objective: To investigate the interaction between FHL₂ and Id proteins and its corresponding epitope. Methods: Interaction between FHL₂ and Id proteins and the corresponding epitope conferring this association were detected by GST pulldown assays. Results: FHL₂ directly interacted with all Id proteins.The second LIM domain containing FHL₂ or N terminal region in Id proteins was essential for the direct binding of FHL₂ to Id proteins. Conclusion: FHL₂ is a novel and broad interacting factor of the Id proteins.Through cooperating with Id proteins,FHL₂ may be involved in multiple biological effects and tumorigenesis mediated by Id proteins.