Abstract: Objective: To search after a kind of new method to improve the bioactivity of hydroxyapatite based biomaterials or coating layer of implants. Methods: A new peptide named P18 with two functional group was synthesized by employing solid phase peptide synthesis and its bioactivity was investigated. Results: Synthetic peptide has high binding ability with hydroxyapatite and cannot be eluted under physiological condition. The peptide can affect the in vitro calcification of gel system and also has TGF-β like activity. The TGF-beta activity was proved by the inhibition of the proliferation of Mv1Lu cell line. It can promote the proliferation of UMR-106 cell line, but the effect is lessened at higher concentration. Under the effect of the peptide, the activity of alkaline phosphatase was higher than control and the time required to reach peak value was shortened. Conclusion: The synthesized peptide possess two functions as design, it may have extensive application prospect as an ingredients of HA based materials or coating layer of implant.