氢氟酸烧伤对肝细胞功能与超微结构的影响
The changes in hepatic structure and function following hydrofluoric acid burns
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摘要: 目的: 探讨氢氟酸烧伤对大鼠肝脏功能与结构的影响及意义。方法: W istar大鼠80只,随机分为氢氟酸烧伤组(A)、等面积烫伤组(B)、大面积烫伤组(C)和对照组(D)。氢氟酸致A组大鼠背部6%总体表面积(TBSA)烧伤,热水致B组和C组大鼠背部Ⅲ度烧伤面积分别为6%TBSA和30%TBSA。分别于伤后5h、10h、24h取血、活杀。测定血标本内ALT、AST、LDH浓度,并通过投射电镜观察肝细胞超微结构的变化。结果: 血清ALT、AST水平变化: A组大鼠伤后各时相点血清ALT和AST浓度均显著高于其它各组(P<0.01)。C组大鼠伤后5h和10h血清ALT和AST显著高于D组与B组动物(P<0.05);血清LDH水平变化: A组大鼠伤后血清LDH水平持续上升,其中5h血清LDH水平显著高于B组及D组动物(P<0.01),伤后10h、24h则显著高于其它各组(P<0.01)。C组大鼠伤后血清LDH水平显著高于B组和D组(P<0.01)。烧伤后肝线粒体形态结构变化: A组与C组大鼠肝细胞超微结构均显示,细胞质中线粒体较多,肿胀呈球形,脊消失。结论: 氢氟酸烧伤后,肝脏损伤不仅较同面积烧伤严重,也较大面积烧伤严重,而肝线粒体形态改变远较同面积烧伤严重,与大面积烧伤接近,提示烧伤复合氟中毒能加重肝细胞结构与功能损伤。Abstract: Objective: To study the changes of hepatic structure and function in rats with hydrofluoric acid burned injury.Methods: Wistar rats were randomly divided into four groups,including group A,B,C and D.The rats of group A and B were received 6% third degree burned injury on the back with hydrofluoric acid or hot water respectively,and those of group C were received 30% TBSA third degree burned injury with hot water.Blood sampling were carried out at 5 hrs,10hrs and 24hrs post burns for examinations of ALT,AST and LDH.The hepatic ultrastructure was investigated with supermicroscope.Results: The levels of serum ALT and AST in rats of group A were significantly increased than that of others（P＜0.01）and the parameter of group C were notably higher than that of group B and D（P＜0.01）.The concentrations of serum LDH of group A were significantly increased than that of group B and D,and higher than that of group C at 10hrs and 24hrs post burns（P＜0.01）.Serum levels of LDH of group C were markedly higher than that of group B and D（P＜0.01）.Mitochondria damages with swelling,vacuolelike changes,and disappearance of carina following hydrofluoric acid and large area burned injury were observed.（Conclusion: The） injuries of the liver indicted by AST,ALT,LDH and the ultrastructure of mitochondria were more seriously post hydrofluoric acid burned injury than those were injured only by hot water. hydrofluoric acid;burns;liver
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