基因枪介导pVAX1-2PFcGB融合肿瘤抗原基因疫苗小鼠体内抑瘤活性研究

Research of antitumor effect of gene gun-mediated DNA vaccine pVAX1-2PFcGB immunization in Balb/c mice

摘要: 目的:探讨基因枪免疫pVAX1-2PFcGB融合肿瘤抗原基因疫苗对小鼠移植瘤生长的抑制作用。方法:在基因枪介导下,向接种EMT6乳腺癌肿瘤细胞的Balb/c小鼠导入融合肿瘤抗原基因疫苗pVAX1-2PFcGB,通过观察计算免疫动物的成瘤时间、肿瘤体积、抑瘤率来评价该基因疫苗的抗肿瘤作用。结果:基因枪介导的pVAX1-2PFcGB融合肿瘤抗原基因疫苗能够明显推迟和抑制移植瘤的生长,显示出明显的抗肿瘤效果。结论:基因枪介导的DNA疫苗pVAX1-2PFcGB能够有效诱导机体的免疫应答,预防和抑制小鼠移植瘤的生长。

Abstract: Objective:To observe the inhibitory effect of fusion gene DNA vaccine pVAX1-2PFcGB immunization with gene gun approaching to subsequent tumor in Balb/c mice.Methods: The Balb/c mice were inoculated with EMT6 cells deriving from Balb/c mice breast cancer and immunized with DNA vaccine pVAX1-2PFcGB by gene gun.The inhibitory effect of DNA vaccine by observing the forming time、volume of tumour and inhibition ratio of tumour were studied.Results: Tumor growth was reduced dramatically.Conclusion: Gene gun-mediated DNA vaccine pVAX1-2PFcGB immunization can induce immunologic response.It is effective and practicable in inhibiting tumor growth.