Abstract: Objective:To establish the method for the observation of human urine.Methods:Atomic force microscope was used to observe nanomedicine.Results:Medicine untreated by nanotechnology were not uniform,and the granules were different in size and shape,the big ones looked as crystals in light microscope,while the small ones were only about 300 nanometers in size.Nanomedicine were found to be uniform in size and shape,the granules were from 100 to 300 nanometers.Conclusion: the results provide the primary studies of nanomedicine may be useful to the further application of nanomedicine in biomedicine.