Abstract: Objective: To evaluate the effects of corneal preservation for lamellar keratoplasty at low temperature. Methods: The corneas （15 human eyes） were preserved under three conditions: Group 1, preserved in glycerine at 4℃, group 2, cryopreserved at -196℃ and group 3 eyeball cryopreserved at -196℃. The ultrastructural changes of the corneas preserved for two years in each group were studied by transmisson electron microscopy.Results: The corneal collagen fibres in the first group were irregularly arranged, loosened and disconnected with difuse fibre degeneration. In the second group, the corneal collageng fibres were regularly arranged with normal electron density. Interfibral spaces were sporadicly dilated with lower electron density. In the third group, the electron density of corneal collagen fibres were uneven with dilated and low electron-densed interfibral spaces. Conclusion: The corneas cryopreserved at -196℃ are ideal materials for optical lamellar keratoplasty. For generally theraputical lamellar keratoplasty, eyeball cryopressrved at -196℃ is recommended, because this method is easy and effective.