Abstract: Objective: C 6 intracerebral and subcutaneous model of Wistar rat was established to study the tumor growing characters and the histological characters.Methods: First group, 10 6 C 6 cells were inoculated into caudate nucleus of 50 Wistar rats and 50 SD rats by stereotaxic method to study the survival time and histological characters respectively Second group,140 Wistar rats were killed in batchs 3,5,7,9,11,13,15 days after intracerebral inoculation,the tumor size,the histological characters were observed Third group, subcutaneous model of 50 Wistar rats was established by inoculation of 2 10 6 C 6 cells,the tumor size,the histological characters were observed.Results: The mean survival time after intracerebral inoculation of Wistar rats and SD rats was （15 9±1 2） days and （18 9±1 8） days respectively,the intracerebral tumor of Wistar ratshow several characteristic features of spontaneus glioblastomas,such as neovasculature,parenchymal invasion,nuclear pleiomorphism and presence of hemorragic and necrotic areas,GFAP and S 100 protein immunohischemistry were positive, the intracerebral tumor of SD rats show there was border between the tumor and cerebral tissue The intracerebral tumor of Wistar rats grew faster during 5 11 day after inoculation The subcutaneous tumor of Wistar rats grew steadily until 25 days after inoculation,then the tumor cease growing and the tumor size decreased Conclusions: C 6 intracerebral model of Wistar rats was more similar to human glioma growing characters, C 6 subcutaneous model of Wistar rat had character of selflimitation