肺增生病变p53基因突变的检测

张鋆歆; 申洪

肺增生病变p53基因突变的检测

张鋆歆,
申洪

Detection of p53 gene mutation in lung preneoplastic lesions

摘要

摘要: 目的：检测p53基因突变率在“肺癌性”增生病变和“肺炎性”增生病变是否存在差异，试图确定“发生真正癌变”的癌前病变，为肺癌前病变的风险性预测提供实验依据。方法：采用PCR-SSCP-DNA测序法，检测了80例“肺癌性”和51例“肺炎性”增生病变的p53基因突变率。结果：“肺癌性”增生组的Ⅰ、Ⅱ、Ⅲ、Ⅳ级增生病变的p53突变率分别为7%(1/15)、33%(4/12)、38%(5/13)、30%(3/10)和“肺炎性”增生组相对应的Ⅰ～Ⅳ级增生病变的突变率7%(1/15)、25%(3/12)、36%(5/14)、40%(4/10)比较无显著性差异(P＞0.05)。结论：p53基因的突变率在两组增生病变没有差别，提示p53基因突变不能作为预测肺增生病变预后的检测指标。

Abstract: Objective: To compare the frequency of p53 gen e mutations in "lung cancerous"hyperplasia with that in"pneumonia" hyperplasia to provide optimal molecular marker for earlier diagonosis of "genuine" preneoplastic lesions Methods: p53 gene mutations in 80 hyperplastic epithelial lesions obtained from patients with squamous cell carcinoma and 51 matched epithelial lesions obtained from patients without lung cancer were screened by polymerase chain reaction single strand conformation polymorphism a nd DNA sequencing analysis Results: No significant difference of p53 mutations was observed between the "lung cancerous" hyperplasia from Ⅰ to Ⅳ grade, which were 7%（1/15)、33%（4/12)、38%（5/13)、30%（3/10) respectively and "pneumonia" matched hyperplasia, which were 7%（1/15)、25%（3/12)、36%（5/14)、40% （4/10) respectively.Conclusion: There is no different rate of p53 mutations between "lung cancerous" hyperplasia and "pneumonia" hyperplasia, and it is suggested that p53 gene would not be an optimal molecular marker for earlier diagonosis of "genuine" preneoplastic lesions

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