Abstract: Objective To investigate the genitourinary mycoplasma infection and drug susceptibility test in our region in order to provide the evidence for rational use of drugs. Methods Clinical symptoms of 9 752 patients with genitourinary mycoplasma infection admitted to our hospital from January 2006-December 2010,and their mycoplasma culture identification and drug susceptibility test were statistically analyzed. Results Of the 9 752 patients,6 215（63.7%） were positive for genitourinary mycoplasma.The main pathogen was mycoplasma urealytium（Uu）.The number of Uu-infected patients tended to increase in the past 5 years during which the positive infection rate was 35.1%,38.2%,47.6%,48.9%,and 49.7%,respectively.The positive infection rate was higher in women than in men.The patients infected with both Uu and Mh were sensitive to doxycycline,minocycline and josamycin but resistant to other drugs（over 56%）. Conclusion Uu and Mh infection can induce genitourinary inflammation.It is necessary for clinical physicians to culture mycoplasma,conduct drug susceptibility tests,and rationally use antibiotics.