神经生长因子对糖尿病大鼠背根神经节神经细丝蛋白变化的影响

The effects of nerve growth factor on the changes of neurofilament in the dorsal root ganglia of diabetic rats

摘要: 目的：观察神经生长因子（NGF）对糖尿病神经病变的治疗作用。方法：采用免疫组织化学染色结合电子计算机图像分析技术，测定糖尿病大鼠背根神经节的结构蛋白-神经细丝蛋白（NF）的相对含量。结果：糖尿病成模后三个月，背根神经节NF的平均灰度值，糖尿病组（DM组）明显低于正常对照组（NC组），NGF组（NGF 200μg·kg^-1·d^-1肌肉注射，30d）虽低于NC组，但却高于DM组，P均＜0.01。结论：NGF可促进感觉神经结构蛋白的表达，有益于糖尿病周围神经病变的恢复

Abstract: Objective: To observe the threapeutic effects of nerver growth factor （NGF） on diabetic peripheral neuropathy.Methods: The changes of the neurofilament （NF） in the dorsal root ganglia（DRG） of diabetic rats were measured by the techniques of immunohistochemistry combing with computerized image analysis system. Results: After inducing the diabetes for three months, the mean gray levels （M.G.L.） of the positive reaction of NF in the DRG were much lower in the DM group than in the normal control group, those in the NGF group （NGF 200 μg·kg^-1·d^-1 IM.×30 d） were lower than in normal control group too, but were higher than in DM group （P＜0.01）.Conclusion: The NGF may promote the expression of the NF in sensory nerve and seems to have the beneficial effects on the recovery of diabetic peripheral neuropathy.