异烟肼、利福平治疗肺结核致肝毒性易感基因的研究

The study on the susceptible gene of isoniazid and rifampicin-induced hepatotoxicity of pulmonary tuberculosis patients

摘要: 目的: 探讨N-乙酰基转移酶(NAT2)基因型与异烟肼、利福平治疗肺结核致肝毒性的相关性。方法: 通过聚合酶链反应限制性片段长度多态性(PCR RFLP)技术分析67例经利福平、异烟肼治疗后发生或未发生肝功能异常的肺结核患者NAT2基因多态性的部位、性质及发生率。结果: 病例组和对照组857位密码子多态性分别是20.3%和7.1%,两组差异显著。结论: NAT2基因型与异烟肼和利福平所致肝毒性关系密切,其中857位密码子点突变可能是结核患者发生肝毒性的易感基因型之一。

Abstract: Objective: To investigate the relationship between the susceptibility of isoniazid and rifampicin-induced hepatotoxicity and the N-acetyltransferase 2（NAT2） genotype in the tuberculosis patients. Methods: NAT2 gene was analyzed in 67 pulmonary tuberculosis patients who had normal or abnormal hepatic function by PCR-RFLP. Results: The frequencies of NAT2 gene mutation at codon 857 in patient group and control group were 20.3% and 7.1%,respectively,there were significant difference. Conclusion: NAT2 gene mutation at codon 857 might be one of susceptible genotype in the tuberculosis patients whose hepatic functions were abnormal.