基于Wnt/β-catenin信号通路探究过表达miR-124对胃癌前病变模型大鼠的干预效果

罗文; 王伟宁; 彭进; 曾微; 姚思; 张嘉怡

doi:10.3969/j.issn.2095-5227.2023.04.013

基于Wnt/β-catenin信号通路探究过表达miR-124对胃癌前病变模型大鼠的干预效果

doi: 10.3969/j.issn.2095-5227.2023.04.013

长沙市第一医院消化内科，湖南长沙　410000

基金项目: 湖南省卫生健康委科研计划课题项目(B2019134)

详细信息

作者简介:
罗文，男，硕士，主治医师。Email: luow198@126.com

通讯作者:
王伟宁，男，博士，主任医师。Email: wango197@126.com

中图分类号: R-332
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出版历程
- 收稿日期: 2022-08-17
- 网络出版日期: 2023-04-10
- 刊出日期: 2023-04-28

Effect of overexpression of miR-124 on gastric precancerous lesion of model rats based on wnt/ β-catenin signaling pathway

Department of Gastroenterology, the First Hospital of Changsha, Changsha 410000, Hunan Province, China

More Information

Corresponding author: WANG Weining. Email: wango197@126.com

摘要

摘要: 背景 Wnt/β-catenin信号通路的活跃可以促进胃黏膜细胞的分裂和增长，增加癌变的可能。目的探究基于Wnt/β-catenin信号通路过表达miR-124对胃癌前病变模型大鼠的干预效果影响。方法选取43只SPF级Wistar大鼠作前瞻性研究，从中选取10只作为对照组(0.9%氯化钠注射液)，并将剩余33只大鼠构建胃癌前病变模型，最终建模成功30只，平均分为模型组(0.9%氯化钠注射液)、miR-124抑制剂组(miR-124 inhibitor)和miR-124模拟物组(miR-124 mimic)。结果与对照组相比，模型组、miR-124抑制剂组、miR-124模拟物组miR-124相对表达量下降，表皮细胞生长因子(epidermal growth factor，EGF)、表皮生长因子受体(epidermal growth factor receptor，EGFR)、白细胞介素-6(interleukin- 6，IL-6)、肿瘤坏死因子α(tumor necrosis factor，TNF-α)水平以及Wnt、β-catenin表达量升高(P均＜0.05)；与模型组相比，miR-124抑制剂组miR-124表达水平下降，EGF、EGFR、IL-6、TNF-α水平以及Wnt、β-catenin表达量升高，miR-124模拟物组miR-124相对表达量升高，EGF、EGFR、IL-6、TNF-α水平以及Wnt、β-catenin表达量降低(P均＜0.05)；与miR-124抑制剂组相比，miR-124模拟物组miR-124相对表达量升高，EGF、EGFR、IL-6、TNF-α水平以及Wnt、β-catenin表达量降低(P均＜0.05)。结论过表达miR-124可降低大鼠胃黏膜的损害，改善其炎症反应，其机制可能与Wnt/β-catenin信号通路被抑制有关。
- 胃癌前病变 /
- miR-124 /
- Wnt/β-catenin信号通路 /
- 胃黏膜 /
- 炎症反应
Abstract: Background Active wnt/β-catenin signaling pathway can promote the division and growth of gastric mucosal cells, and increase the possibility of carcinogenesis. Objective To explore the effect of overexpression of miR-124 on precancerous lesions of gastric cancer rats based on wnt/β-catenin signaling pathway. Methods Forty-three SPF Wistar rats were selected for a prospective study, 10 of which were selected as the control group (normal saline), and the remaining 33 rats were used to construct a model of gastric precancerous lesions, with the successful modeling of 30 rats. They were divided into model group (saline), miR-124 inhibitor group and miR-124 simulator group. Results Compared with the control group, the relative expression of miR-124 in the model group, the miR-124 inhibitor group and the miR-124 simulator group decreased, while the epidermal growth factor (EGF), epidermal growth factor receptor (EGFR), interleukin-6 (IL-6), tumor necrosis factor, the levels of TNF-α, wnt and β-catenin were increased (P <0.05); Compared with the model group, the expression level of miR-124 in the miR-124 inhibitor group was decreased, while the expression levels of EGF, EGFR, IL-6, TNF-α, wnt and β-catenin were increased, and the relative expression level of miR-124 in the miR-124 simulator group was also increased. The levels of EGF, EGFR, IL-6, TNF-α, wnt and β-catenin were decreased (all P <0.05). Compared with the miR-124 inhibitor group, the relative expression level of miR-124 in the miR-124 simulator group was increased, while the levels of EGF, EGFR, IL-6, TNF-α, wnt and β-catenin were decreased (all P <0.05). Conclusion Overexpression of miR-124 can reduce the damage of gastric mucosa in rats and improve its inflammatory response. The mechanism may be related to inhibition of wnt/ β- catenin signal pathway.
- pregastric cancer lesions /
- miR-124 /
- Wnt/β-catenin /
- gastric mucosa /
- inflammatory response

HTML全文

图 1 各组大鼠胃病理组织学观察(HE染色，200×)

A：对照组；B：模型组；C：miR-124抑制剂组；D：miR-124模拟物组

Figure 1. Pathological histological observation of rats' stomach tissues (HE staining, 200×)

A: control group; B: model group; C: miR-124 inhibitor group; D: miR-124 simulator group

下载: 全尺寸图片幻灯片

图 2 Wnt/β-catenin信号通路蛋白Western blot图

Figure 2. Western blot of Wnt/β -catenin signaling pathway protein

下载: 全尺寸图片幻灯片

表 1 各组大鼠肾组织miR-124相对表达量对比

Table 1. Comparison of miR-124 expression in kidney tissues

指标	对照组(n=10)	模型组(n=10)	miR-124抑制剂组(n=10)	miR-124模拟物组(n=10)	F值	P值
miR-124相对表达量	6.82 ± 0.88	2.65 ± 0.43^a	1.34 ± 0.15^ab	4.94 ± 0.92^abc	13.46	＜0.001
^aP＜0.05，vs 对照组；^bP＜0.05，vs 模型组；^cP＜0.05，vs miR-124抑制剂组。

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表 2 各组大鼠血清标志物水平对比

Table 2. Comparison of serum biomarkers levels in each group

指标	对照组(n=10)	模型组(n=10)	miR-124抑制剂组(n=10)	miR-124模拟物组(n=10)	F值	P值
EGF/(ng·mL^-1)	0.69 ± 0.12	3.02 ± 0.29^a	4.62 ± 1.03^ab	1.48 ± 5.97^abc	11.98	＜0.001
EGFR/(pg·mL^-1)	1.50 ± 0.72	3.41 ± 1.39^a	4.95 ± 1.62^ab	2.04 ± 6.02^abc	6.154	＜0.001
IL-6/(pg·mL^-1)	31.61 ± 4.25	60.78 ± 6.29^a	71.42 ± 7.03^ab	42.48 ± 5.07^abc	15.32	＜0.001
TNF-α/(pg·mL^-1)	32.37 ± 4.02	68.41 ± 6.19^a	84.05 ± 8.62^ab	51.14 ± 6.02^abc	17.18	＜0.001
^aP＜0.05，vs 对照组；^bP＜0.05，vs 模型组；^cP＜0.05，vs miR-124抑制剂组。

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表 3 各组大鼠Wnt/β-catenin通路蛋白表达量对比

Table 3. Comparison of wnt / β -catenin pathway protein expression in rats

指标	对照组(n=10)	模型组(n=10)	miR-124抑制剂组(n=10)	miR-124模拟物组(n=10)	F值	P值
Wnt	1.00 ± 0.01	3.02 ± 0.49^a	4.85 ± 0.55^ab	1.81 ± 0.32^abc	6.538	＜0.001
β-catenin	1.00 ± 0.01	3.57 ± 0.48^a	4.76 ± 0.59^ab	2.03 ± 0.21^abc	9.295	＜0.001
^aP＜0.05，vs 对照组；^bP＜0.05，vs 模型组；^cP＜0.05，vs miR-124抑制剂组。

下载: 导出CSV

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