食管癌Ⅰ型纤溶酶原激活物抑制剂的表达研究

Expression of PAI-1 plasminogen activator inhibitor-1 in esophageal carcinoma

摘要: 目的: 探讨食管癌组织中Ⅰ型纤溶酶原激活物抑制剂(PAⅠ-1)的表达特征。方法: 采用定量的逆转录聚合酶链反应(RT-PCR)方法检测基因的表达。结果: 食管癌组织中PAⅠ-1的mRNA的表达水平显著高于正常粘膜组织(P<0.0 1),组织原位杂交显示PAⅠ-1的mRNA主要分布于肿瘤细胞的胞浆中。结论: PAⅠ-1的mRNA在食管癌中的表达上调,RT-PCR的定量分析可用于肿瘤诊断的辅助方法。

Abstract: Objective: To detect the plasminogen activator inhibitor 1 （PAⅠ-1） gene expression in tissue of esophageal carcinoma.Methods: Quantitative reverse transcription polymophoase chain reaction （PT PCR） has been used to detect PAⅠ-1 gene expression.Results: The mRNA level of PAⅠ-1 in esophageal carcinoma is higher than those in nucosa tissue surrounding carcinoma.Conclusion: The level of PAⅠ-1 mRNA expression increases significantly in esophageal carcinoma tissues, the detection of PAⅠ-1 gene by RT PCR in esophageal carcinoma tissues may provid the new diagnosis methods for esophageal carcinoma.