腹部开放伤合并海水浸泡致犬急性心力衰竭实验模型建立
Establishment of experimental model of acute heart failure in dogs with open abdominal injury induced by sea water immersion
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摘要: 目的 建立犬腹部开放伤合并海水浸泡致犬急性心力衰竭模型。方法 23只成年杂种犬分为腹部开放伤合并海水浸泡组15只,诱导急性心力衰竭(A组),单纯腹部开放伤(B组)8只。A组于致伤前后、浸泡海水即刻、0.5h、1h、2h,观察急性左心衰发生的情况,检测血液动力学变化,超声检查心功能,采血检查BNP,B组按照A组相同的时间点做上述同样检查。结果 犬腹部开放伤合并海水浸泡成功诱导急性心力衰竭8只(A组),成功率53.3%,在浸泡后心率、收缩压、舒张压、平均动脉压、中心静脉压均较伤前及B组下降,至浸泡2h时,肺动脉嵌压为(25±2.3)mmHg(1mmHg=0.133kPa),心输出量较伤前减低46.67%,较B组降低48.39%(P<0.01),心指数、左室舒张末容积、左室收缩末容积、每搏输出量、左室射血分数均较伤前和B组减低(P<0.05),脑利钠肽为(622.77±52.21)pg/mL,较致伤前升高441.6%,较B组升高302.8%,与致伤前及B组相比均显著升高(P<0.001)。结论 犬腹部开放伤经海水浸泡后可成功建立急性心力衰竭模型,浸泡2h时成功率为53.3%。Abstract: Objective To establish an experimental model of acute heart failure（AHF）in dogs with open abdominal injury induced by sea water immersion. Methods Twenty-three healthy adult dogs were divided into open abdominal injury group（n=15）as group A and AHF model group n=8)as group B.After 0.5,1 and 2h of sea water immersion in group A,AHF was induced,and hemodynamic change in groups A and B was detected by sonography and BNP. Results AHF was induced in 8 dogs（53.3%）in group A.After sea water immersion,the heart rate,SBP,SDP,MAP,CVP were significantly higher in group A than in group B.Two hours after sea water immersion,the PAWP was 25±2.3mmHg,and the CO was 46.67%and 48.39%in group A,which were higher than those in group B（P＜0.01）.The levels of LVEDV,LVESV,SV,and LVEF were significantly higher in group A than in group B（P＜0.05）.The BNP was 622.77±52.21pg/mL in group A,which was increased by 441.6%compared with before injury and was 302.8% higher than in group B（P＜0.001）. Conclusion Open abdominal injury induced by sea water immersion can be used to establish the model of AHF.
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