Abstract: Objective: Through culture of monotony nucleus cells in bone marrow and cytokines in vitro, to study cytokines activating lymphocytes in bone marrow and effecting stem or blast cells in bone marrow.Methods: To combine IL 1,IL 2,γ IFNand CD3antibody into groups of IL 2,CD3 AKand CIK And to culture with monotony nucleus cells in bone marrow To observe shape of cells and to examine activated immunocytos and preservation of stem cells.Results: The cell numbers of controlling group decreased, those of IL 2 group without variation Cell numbers and cell toxicity of CD3 AK and CIK group increased obviously, and preservations of stem cells are about 60%～87%.Conclusion: Culture with IL 2 or CD3 antibody and monotony nucleus cells in bone marrow can stimulate immunocyto proliferation and reserve sufficient stem cells