反相离子对高效液相色谱法测定大鼠缺血再灌注心肌ATP的含量

Determination of ATP in the rat heart with ischemia reperfusion by ion-pair reversed-phase HPLC

摘要: 目的: 研究大鼠心肌缺血再灌注损伤后三磷酸腺苷（ATP）的含量的变化。方法: 应用反相离子对高效液相色谱法（HPLC）分离测定,流动相为0.1 mmol/L KH₂PO₄+8 mmol/L氢氧化四丁铵,检测波长259nm。结果: ATP标准液与峰面积呈良好的线性关系（r=0.998）。重复测定不同浓度ATP标准液精密度（变异系数）1.91%~5.78%。回收率为97.25%~100.05%。结论: 反相离子对高效液相色谱法可以准确测定大鼠心肌组织ATP的含量。

Abstract: Objective: To study the change alteration of ATP in the rat heart with ischemia reperfusion injury by lon-pair reversed-phase HPLC. Methods: Ion-pair reversed-phase HPLC methods was developed for determination of ATP.The solvent consist of 0.1 mmol·L^-1 KH₂PO₄;8 mmol·L^-1 tetrabutylammonium hydrogen.The separation was monitored at 259nm.（Results: The） calibration curve showed good linearity（r=0.998）.The coefficients of variation（C.V） were estimated from（repeated） determinations of standard ATP.The intraassay C.V varied from 1.91% to 5.78%.The recovery was between 97.25% and 100.05%. Conclusions: This methods is precise,it can be used for determination of ATP in the rat heart.