Abstract: Objective: The aim of this study was to evaluate the efficiency of domestic expanded polvtetrafluoroethylene （e-PTFE） membrane on periodontal guided tissue regeneration （GTR）. Methods: The periodontal defects of 10 dog teeth treated with e-PTFE were investigated for three monthes, and compared with the contrals （7 teeth）. Results: The periodontal guided tissue regeneration in the treated group were significant higher than those in the control group （P＜0.05）, but they did not recovered to the clinical attachment level at the end of third month. Conclusion: Our study indicatis that e-PTFE membranes seem to be possible to produce the GTR in the replacement of periodontal defects.