Role of microRNA-25 in invasion and metastasis of laryngeal squamous cell carcinoma cells
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摘要:
背景 导致喉癌患者死亡的主要原因是复发和转移，喉癌侵袭转移的分子机制已成为当前研究的重点。 目的 研究microRNA-25(miR-25)在喉鳞癌Hep-2细胞体外侵袭转移中的调控作用。 方法 应用微小RNA过表达载体(mimic)和抑制载体(inhibitor)转染Hep-2细胞，分别增加和抑制Hep-2细胞中miR-25的表达，Transwell法和细胞划痕实验检测Hep-2细胞侵袭迁移能力的变化，MTT法检测细胞增殖能力变化，绘制细胞生长曲线。 结果 上调Hep-2细胞中miR-25表达后，Transwell实验和细胞划痕实验显示Hep-2细胞的体外侵袭和迁移能力显著低于正常对照组(P＜0.01)，MTT检测结果显示过表达miR-25组Hep-2细胞增殖能力亦显著降低。而抑制miR-25表达后，Hep-2细胞体外侵袭、迁移和增殖能力均显著增强。 结论 miR-25的表达量显著影响喉鳞癌Hep-2细胞体外侵袭、迁移和增殖能力，提示miR-25可能是抑制喉鳞癌转移的一个有效靶点。 -
关键词:
- 喉鳞癌Hep-2细胞系 /
- microRNA-25 /
- 细胞转染 /
- 细胞侵袭和迁移 /
- 细胞增殖
Abstract:Background Recurrence and metastasis are the main causes of death in patients with laryngeal cancer. The molecular mechanisms of invasion and metastasis of laryngeal cancer have become the focus of current research. Objective To study the regulatory role of microRNA-25 in the invasion and metastasis of laryngeal squamous cell carcinoma cells (Hep-2) in vitro. Methods Hep-2 cells were transfected with microRNA overexpression vector (mimic) or inhibitor vector (inhibitor) to increase or inhibit the expression of microRNA-25, respectively. The changes of invasion and migration ability of Hep-2 cells were detected by Transwell method and cell scratch test. The changes of cell proliferation ability were detected by MTT method, and the cell growth curve was drawn. Results After overexpression of microRNA-25 in Hep-2 cells, Transwell test and cell scratch test showed that the invasion and migration of Hep-2 cells in vitro were significantly lower than those in the normal control group (P < 0.01, respectively). MTT assay showed that the proliferation of laryngeal cancer cells in the experimental group decreased significantly. After inhibiting the expression of microRNA-25, the invasion, migration and proliferation of Hep-2 cells in vitro increased significantly. Conclusion Overexpression of microRNA-25 can significantly inhibit the invasion, migration and proliferation of laryngeal squamous cell carcinoma Hep-2 cells in vitro, suggesting that microRNA-25 may be an effective target to inhibit the metastasis of laryngeal squamous cell carcinoma. -
图 1 RT-qPCR检测Hep-2细胞转染miR-25 mimic和miR-25 inhibitor后miR-25表达变化(aP＜0.05，bP＜0.01，vs Control )
Figure 1. RT-qPCR detection of miR-25 expression after transfection of miR-25 mimic and miR-25 inhibitor (aP＜0.05, bP＜0.01, vs control)
图 2 Hep-2细胞Transwell侵袭实验(bP＜0.01，vs Control)
Figure 2. Transwell cell migration test of Hep-2 cells (bP＜0.01, vs control)
图 3 Hep-2细胞划痕实验(bP＜0.01，vs Control)
Figure 3. Cell scratch test of Hep-2 cells (bP＜0.01, vs control)
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