Abstract:
Background Recurrence and metastasis are the main causes of death in patients with laryngeal cancer. The molecular mechanisms of invasion and metastasis of laryngeal cancer have become the focus of current research.
Objective To study the regulatory role of microRNA-25 in the invasion and metastasis of laryngeal squamous cell carcinoma cells (Hep-2) in vitro.
Methods Hep-2 cells were transfected with microRNA overexpression vector (mimic) or inhibitor vector (inhibitor) to increase or inhibit the expression of microRNA-25, respectively. The changes of invasion and migration ability of Hep-2 cells were detected by Transwell method and cell scratch test. The changes of cell proliferation ability were detected by MTT method, and the cell growth curve was drawn.
Results After overexpression of microRNA-25 in Hep-2 cells, Transwell test and cell scratch test showed that the invasion and migration of Hep-2 cells in vitro were significantly lower than those in the normal control group (P < 0.01, respectively). MTT assay showed that the proliferation of laryngeal cancer cells in the experimental group decreased significantly. After inhibiting the expression of microRNA-25, the invasion, migration and proliferation of Hep-2 cells in vitro increased significantly.
Conclusion Overexpression of microRNA-25 can significantly inhibit the invasion, migration and proliferation of laryngeal squamous cell carcinoma Hep-2 cells in vitro, suggesting that microRNA-25 may be an effective target to inhibit the metastasis of laryngeal squamous cell carcinoma.
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