Abstract: Objective: To investigate the composition of pathogenic bacteria in neonatal septicemia and the antimicrobial resistance of these bacteria in recent years and this region and to guide the clinical treatment. Methods: To make an analysis of clinical data of 108 patients between Jan.2001 and Dec.2004. Results: All 108 cases are single bacterium,including 61 coagulase negative staphylococci（CNS）,25 Staphylococcus aureus,10 colibacillus,5 enterococcus.The antimicrobial resistance of staphylococcus to penicillin or ampicillin or erythromycin was all above 90%.The sensitive drugs were vancomycin,rifampicin,chloramphenicol and fluoroquinolone.The antimicrobial resistance of gram-negative bacillus to ampicillin was 100%.while sensitive drugs were imipenem,aztreonam,cefepime and amikacin. Conclusion: CNS was the main pathogenic bacterium in neonatal septicemia at present and in the region,the most knotty problem was the infection of neonate caused by many antimicrobial resistant bacteria. Medication should follow the result of surveillance of antimicrobial resistance.