Abstract: Objective To study the relation of different concentrations and culture time of esophageal cancer cells with their ultra-weak photon emission intensity. Methods Ultra-weak photon emission of esophageal cancer cells was detected using the biological ultra-weak emission detection techniques. Results The concentration of esophageal cancer cells influenced the intensity of ultra-weak photon emission. The intensity of ultra-weak photon emission was different at different culture times of esophageal cancer cells and reached its peak at 72 hours. Analysis of ultra-weak photon,emission spectra distribution showed that the greatest intensity of ultra-weak photon emission was observed at 535nm of esophageal cancer cells with their ultra-weak photon emission intensity. Conclusion The ultra-weak photon emission of esophageal cancer cells depends on their different concentrations and culture times.